Hybrid Genome Assembly and Comparative Genomics of Brucella anthropi

Published:

This mini project focuses on the hybrid genome assembly and comparative genomics analysis of Brucella anthropi using both Oxford Nanopore long-read sequencing and Illumina short-read sequencing. The goal was to generate a high-quality genome assembly, improve accuracy through polishing, and perform comparative genomic analysis for functional annotation and antimicrobial resistance screening.

  • Performed hybrid genome assembly using Oxford Nanopore + Illumina sequencing data
  • Used Trycycler for consensus assembly generation
  • Applied polishing with Medaka and Polypolish for improved genome accuracy
  • Evaluated assembly quality using BUSCO and QUAST
  • Conducted genome annotation using Bakta and Prokka
  • Screened antimicrobial resistance genes using AMRFinderPlus and Abricate

Key Results:

  • Genome Size: 5.10 Mb
  • Number of Contigs: 5
  • N50 Value: 2.88 Mb
  • GC Content: 56%
  • BUSCO Completeness: 99.5%
  • Protein-Coding Genes Identified: 5,325

Full Report: Download Full Report (PDF)

Repository:

  • _https://github.com/Sudu-09-Nep/ont-illumina-hybrid-assembly
  • https://github.com/Sudu-09-Nep/Brucella_phylo